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KMID : 0545120020120010157
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Journal of Microbiology and Biotechnology
2002 Volume.12 No. 1 p.157 ~ p.161
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Isolation of Lactococcus lactis Strain with β-Galactosidase Activity from Kimchi and cloning of lacZ Gene from the Isolated Strain
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Park, Rae Jun
Lee, Kwang Hee/Kim, Su Jung/Park, Jae Yong/Nam, Su Jin/Yun, Han Dae/Lee, Hyong Joo
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Abstract
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A lactic acid bacteria with β-gal activity was isolated from Kimchi, a traditional fermented vegetable food in Korea. The isolate was identified as a Lactococcus lactis strain and named L. lactis A2. The gene encoding β-gal of L. lactis A2 was cloned as a 5.8 kb Pstl fragment. DNA sequencing identified the complete lacA (galactoside acetyltransferase)-lacZ (β-galactosidase) genes together with the 3´ part of upstream galT (galactose-l-phosphate uridyltransferase), and the 5´ region of downstream galE (UDP-galactose-4-epimerase) genes. L. lactis A2 had the same gal/lac operon structure as in L. lactis subsp. lactis 7962. Other genes of the Leloir pathway are most likely to be located in the 5´ upstream of the 5.8 kb fragment on the A2 chromosome. Sequences downstream of galE were different from those of L. lactis subsp. lactis 7962.
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